Bioinformatics For Next Generation Sequencing Technologies
About Course
Course Overview
Current state-of-the-art bioinformatics techniques for processing, quality control, analysis and interpretation of high-throughput next generation sequencing data from DNA fragment alignment to variant calling and phenotype prediction.
What You Will Learn
- NGS platforms and sequencing technologies (Illumina, PacBio, Nanopore)
- Raw data quality assessment with FastQC and MultiQC
- Adapter trimming and read filtering
- Reference genome alignment strategies
- De novo assembly approaches
- RNA-seq differential expression analysis
Prerequisites
Basic molecular biology and genetics knowledge. Familiarity with Linux command line.
Duration
8 weeks, 3-4 hours per week
Level
Intermediate