Bioinformatics For Next Generation Sequencing Technologies

Course Overview

Current state-of-the-art bioinformatics techniques for processing, quality control, analysis and interpretation of high-throughput next generation sequencing data from DNA fragment alignment to variant calling and phenotype prediction.

What You Will Learn

• NGS platforms and sequencing technologies (Illumina, PacBio, Nanopore)
• Raw data quality assessment with FastQC and MultiQC
• Adapter trimming and read filtering
• Reference genome alignment strategies
• De novo assembly approaches
• RNA-seq differential expression analysis

Prerequisites

Basic molecular biology and genetics knowledge. Familiarity with Linux command line.

Duration

8 weeks, 3-4 hours per week

Level

Intermediate